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KMID : 0880220120500060994
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Journal of Microbiology
2012 Volume.50 No. 6 p.994 ~ p.1002
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Catabolite control protein a of Streptococcus suis type 2 contributes to sugar metabolism and virulence
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Yulong Tang
Wei Wu
Xiaoyan Zhang
Zhongyan Lu
Jianshun Chen
Weihuan Fang
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Abstract
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Catabolite control protein A (CcpA) is the major transcriptional regulator in carbon catabolite repression in several Gram-positive bacteria. We attempted to characterize the role of a CcpA homologue of Streptococcus suis type 2 in sugar metabolism and virulence. Addition of glucose or sucrose to the defined medium significantly reduced the activity of raffinose-inducible α-galactosidase, cellobiose-inducible β-glucosidase, and maltose-inducible α-glucosidase of the wild-type strain by about 9, 4, and 2-3 fold, respectively. Deletion of ccpA substantially derepressed the effects of repressing sugars on α-galactosidase or β-glucosidase activity. The ccpA deletion mutant showed reduced expression of virulence genes sly and eno (P<0.05), decreased adhesion to and invasion into endothelial cells (P<0.05), and attenuated virulence to mice with significant reduction of death rate and bacterial burden in organs, as compared to the wild-type strain. Both the in vitro and in vivo defect phenotypes were reversible by ccpA complementation. Thus, this study shows that CcpA of S. suis type 2 plays an important role in carbon catabolite repression and virulence.
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KEYWORD
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Streptoccoccus suis type 2, catabolite control protein A, carbon catabolite repression, virulence
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